Fig. 5: SIDT2 exhibits ceramidase activity. | Nature Communications

Fig. 5: SIDT2 exhibits ceramidase activity.

From: Structural insight into the human SID1 transmembrane family member 2 reveals its lipid hydrolytic activity

Fig. 5

a The ceramidase activity was detected in the wild-type SIDT2 sample but not in the H796A-H800A mutant sample. The SIDT2 alone condition and the reaction buffer plus ceramide (d18:1/18:0) (C18) condition serve as controls. Detected sphingosine values are represented as the mean ± s.d. (standard deviations) of three independent measurements (n = 3). Source data are provided as a Source Data file. b Michaelis-Menten analysis of the SIDT2 ceramidase activity. Detected sphingosine values are represented as the mean ± s.d. of three independent measurements (n = 3). Source data are provided as a Source Data file. c Schematic illustration of the hydrolysis of ceramide by SIDT2.

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