Fig. 2: Switch peptide rearrangement leads to gain of functionality.
From: Activating hidden signals by mimicking cryptic sites in a synthetic extracellular matrix
A Schematic illustration of HUVEC cells in culture with fluorescently labeled switch peptide (E5G2KSYIGR-KRB). Upon activation by trypsin, the peptide rearranges into a fluorescently labeled functional peptide, YIGSRKRB, where the YIGSR epitope binds to cell surface integrins. Peptide E5G2KSYIGR-KRB is also fluorescent, but it cannot bind to integrins and is removed in the washing step. B Chemical structure of fluorescently labeled switch peptide. C Brightfield, fluorescence, and merged images of HUVECs preincubated with Ac-E5G2KSYIGR-KRB and trypsin at various timepoints, and positive control Ac-YIGSR-KRB at 30 h. Scale bar = 100 µm. D Corrected total cell fluorescence (CTCF) values at each timepoint measured from fluorescence images. Statistical tests are included in Supplementary Table 1 with n = 3 independent experiments and 5 replicates per experiment. Data are presented as mean values ± SD.
