Fig. 3: Paneth cell and antimicrobial peptide deficiency in TVP23B-deficient small intestine. | Nature Communications

Fig. 3: Paneth cell and antimicrobial peptide deficiency in TVP23B-deficient small intestine.

From: Trans-Golgi protein TVP23B regulates host-microbe interactions via Paneth cell homeostasis and Goblet cell glycosylation

Fig. 3

a Representative periodic acid Schiff (PAS) staining and electron microscopy from Tvp23b+/+ and Tvp23b−/− terminal ileums. b Lysozyme immunofluorescent staining of Tvp23b+/+ and Tvp23b−/− tissue. c Representative electron microscopy from Tvp23b+/+ and Tvp23b−/− terminal ileums. d Composite plot of relative abundance as determined by mass spectrometry from small soluble peptide fraction of Tvp23b+/+ and Tvp23b−/− terminal ileums (n = 3 for each genotype). Normalized protein level [mutant sample/(mutant sample) + (HET sample)] is plotted on the Y-axis. Points at Y = 1 denote proteins exclusively identified in the mutant sample; points at Y = 0 denote proteins exclusively identified in the HET sample. Protein abundance correlates with spectral count e Relative mRNA expression of Paneth cell specific genes, alpha-defensin-1, alpha-defensin-4, alpha-defensin-5, alpha-defensin-21, alpha-defensin-29, lysozyme1 (n = 4 ileal samples from independent mice; ***P < 0.001, ****P < 0.0001). Data are expressed as means ± s.d. and significance was determined by unpaired Student t-test (e). Data is representative of at least 3 independent experiments.

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