Fig. 6: Glycosylation defect of Tvp23b−/− colonocytes. | Nature Communications

Fig. 6: Glycosylation defect of Tvp23b−/− colonocytes.

From: Trans-Golgi protein TVP23B regulates host-microbe interactions via Paneth cell homeostasis and Goblet cell glycosylation

Fig. 6

a Representative staining of mucous layer from Tvp23b+/- and Tvp23b−/− colons. b Proteomic analysis of colonic mucus layer composition. c Muc2 concentration as determined per femtoliter of mucus. (n = 5 independent mice per group) d Immunoblot of Mucin-2 in colonocytes from 3 separate Tvp23b+/− and Tvp23b−/− animals. e Representative core linkage analysis of O-glycans in Tvp23b+/+ and Tvp23b−/− colonocytes from three separate experiments with two mice per genotype. Data (c) are expressed as means ± s.d. and significance was determined by unpaired Student t-test.

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