Fig. 5: Sequence comparison of SL3 RNA elements in betacoronavirus genomes and their binding abilities to human TIA1 protein. | Nature Communications

Fig. 5: Sequence comparison of SL3 RNA elements in betacoronavirus genomes and their binding abilities to human TIA1 protein.

From: Mutagenesis and structural studies reveal the basis for the specific binding of SARS-CoV-2 SL3 RNA element with human TIA1 protein

Fig. 5

a Multiple sequence alignment showing nucleotide coverage within SL3 RNA elements of representative species within genus Betacoronavirus. The transcriptional regulatory sequences (TRS) are highlighted by red box. Six identified sites important for SARS-CoV-2 SL3 binding by TIA1 protein are marked by red triangles. Sequence positions of SL3 RNA element in each species are given in parentheses. BatHp, Bat Hp-betacoronavirus/Zhejiang2013; RousettusBat, Rousettus bat coronavirus; RousettusBatHKU9, Rousettus bat coronavirus HKU9; PipistrellusBat, Pipistrellus bat coronavirus HKU5; TylonycterisBat, Tylonycteris bat coronavirus HKU4; England1, Betacoronavirus England 1; MERS, Middle East respiratory syndrome-related coronavirus; Erinaceus, Betacoronavirus Erinaceus/VMC/DEU/2012. The binding curves between human TIA1 protein and SL3 RNA elements from (b) RousettusBatHKU9 and (c) MERS genomes. The Kd values were calculated from the EMSA image quantification from three independent experiments. Data are presented as mean ± SD. Source data are provided as a Source Data file.

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