Fig. 2: Restoration of iNKT development in Trav11-Traj18-Trac transgenic mice.
From: Tnpo3 controls splicing of the pre-mRNA encoding the canonical TCR α chain of iNKT cells
a Flow cytometric profiles of total thymocytes isolated from the animals of the indicated genotypes stained with anti-CD4 and anti-CD8 antibodies (upper row) and anti-CD3 antibodies and an αGalCer-CD1d tetramer (bottom row). The profiles are representative of three animals each; the percentages of CD4, CD8, and iNKT cells are indicated. b Number of iNKT cells in mice of the indicated groups (genotype designation is indicated in a separate panel); n = 3 biological replicates for genotype a; n = 5 biological replicates for genotype b; n = 7 biological replicates for genotype c; n = 4 biological replicates for genotype d; mean ± s.e.m. are shown. c Variable CD4/CD8 cell ratios in mice of the indicated groups (genotype designation is indicated in a separate panel). The reduced CD4/CD8 ratio is indicative of precocious expression of the transgene; in the absence of Tnpo3, the reduced CD8+ thymocyte numbers skew the ratio in the opposite direction (c.f. Fig. 1c); n = 3 biological replicates for genotype a; n = 5 biological replicates for genotype b; n = 7 biological replicates for genotype c; n = 4 biological replicates for genotype d; mean ± s.e.m. are shown. Source data are provided as a Source Data file.
