Fig. 6: Mechanism of impaired splicing of Trav11-Traj18-Trac transcripts in Tnpo3-deficient thymocytes.

a Ratio of expression levels of Srsf and Hnrnp gene family members in DP thymocytes of the two indicated genotypes; each dot represents a single gene; n = 11 Srsf genes; n = 19 Hnrnp genes. Single sample t-test, two-tailed; hypothetical mean = 1 to indicate no change between the two genotypes. The expression levels of Srsf and Hnrnp gene family members in DP thymocytes of the two genotypes are individually depicted in Supplementary Fig. 5e. Numbers of Hnrnpa1 binding sites in the introns of Trav genes (b) and downstream of Traj genes (c). Only two Trav genes possess two Hnrnpa1 binding sites, one of which is Trav11 (red); only three Traj genes posses an Hnrnpa1 binding site downstream of the splice donor site, one of which is Traj18 (red). The expression levels of these genes are depicted in Supplementary Fig. 5d. d Increased expression of Hnrnpa1 in Tnpo3-deficient thymocytes as determined by qPCR; the ratios of expression levels in iNKT cells and CD3⁺ thymocytes are shown; n = 3 biological replicates for both conditions; mean ± s.e.m. are shown. e Expression levels of unspliced endogenous Trav11-Traj18-Trac transcripts in DP thymocytes of the indicated genotypes as determined by qPCR; n = 3 biological replicates for both conditions; mean±s.e.m. are shown. The locations of Hnrnpa1 binding sites in the transcript is schematically indicated at the top. a, d, e, t-test, two-tailed. Source data are provided as a Source Data file.