Fig. 4: phactor™ facilitates ultraHTE direct-to-biology campaigns.
From: Rapid planning and analysis of high-throughput experiment arrays for reaction discovery
a Event workflow for performing ultraHTE using phactor™ and a Mosquito robot. b Design of 1280 well amide coupling plate. 80 amines were selected to react with carboxylic acid 26. Eight conditions were run in duplicate for each amine. c Results of the amide coupling are shown as a product/internal standard integration ratio from a 2-min LCMS injection of each well. The Mosquito robot is utilized to split the size 1536 plate into four sizes 384 plates for LCMS and bioassay analysis. d Percent inhibition of SARS-CoV-2 MPro when treated with a sample of the reaction mixture from the corresponding well. The 1280 plate is visually recreated. e IC50 curves for three scaled-up compounds chosen from the reaction array. Compounds 27–29 display a range of assay and inhibitory responses.
