Fig. 3: Full-length MBD2a partially rescues the differentiation block in Mbd3 KO ESCs. | Nature Communications

Fig. 3: Full-length MBD2a partially rescues the differentiation block in Mbd3 KO ESCs.

From: Dissecting the roles of MBD2 isoforms and domains in regulating NuRD complex function during cellular differentiation

Fig. 3: Full-length MBD2a partially rescues the differentiation block in Mbd3 KO ESCs.The alternative text for this image may have been generated using AI.

a Flow cytometry analysis indicating the number of live cells (left, WT to Mbd3 KO p = 0.0003, Mbd3 KO to +MBD2b p = 0.0091), CD24+ (middle, WT to Mbd3 KO p = 0.0002, Mbd3 KO to +MBD2b p = 0.0091), and CD24 + CD56+ (right, WT to Mbd3 KO p = 0.0001, Mbd3 KO to +MBD2b p = 0.0070) NPCs at day8 of neuronal differentiation of WT, Mbd3 KO, or Mbd3 KO stably expressing MBD2b, MBD2a or MBD2t. Each data point represents individual cell lines. For each cell line 3 independent clones were analyzed (biological replicates), except for MBD2t, where three technical replicates of one clone were analyzed. Error bars represent mean +/− SD. p values were calculated using an unpaired, two-tailed t test (Mann-Whitney). Source data is provided as a Source Data file. Microscopy images of in vitro derived neurons from Mbd3 KO stably expressing MBD2a (b) or MBD2t (c) at 20× magnification. Similar results were obtained from 3-5 independent replicates. d Flow cytometry analysis indicating the number of live cells (left, WT to +MBD2a p = 0.0016, +MBD2a to +MBDaΔGR p = 0.0012), CD24+ (middle, WT to +MBD2a p = 0.0011, +MBD2a to +MBDaΔGR p = 0.0040), and CD24 + CD56+ (right, WT to +MBD2a p = 0.0004, +MBD2a to +MBDaΔGR p = 0.0002) NPCs at day8 of neuronal differentiation of WT or Mbd3 KO stably expressing MBD2a, MBD2aΔGR or MBD2aR191C. Each data point represents individual cell lines. For each cell line 3 independent clones were analyzed, n = 2 biological replicates. Error bars represent mean +/− SD. p values were calculated using an unpaired, two-tailed t test (Mann-Whitney). Source data is provided as a Source Data file. e Microscopy images of in vitro derived neurons from Mbd3 KO stably expressing MBD2aΔGR or MBD2aR191C at 20× magnification. Similar results were obtained from 3-5 independent replicates.

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