Fig. 3: Comparison of the retrograde transduction tropism of AAV11 and AAV2-retro after PAG injection. | Nature Communications

Fig. 3: Comparison of the retrograde transduction tropism of AAV11 and AAV2-retro after PAG injection.

From: AAV11 enables efficient retrograde targeting of projection neurons and enhances astrocyte-directed transduction

Fig. 3: Comparison of the retrograde transduction tropism of AAV11 and AAV2-retro after PAG injection.

a Connectivity diagram shows the injection site (periaqueductal gray, PAG) and its upstream areas in ectorhinal area (ECT) and ventromedial hypothalamic nucleus (VMH). AAV11-EF1α-EGFP and AAV2-retro-EF1α-mCherry viruses were mixed at a particle ratio of 1:1 (1.8 × 109 VG in total) and injected into PAG of C57BL/6 mice. Diagram was created with BioRender.com. b Fluorescence distribution of EGFP (AAV11) and mCherry (AAV2-retro) at the PAG injection site. Scale bar = 200 μm. c EGFP (AAV11) and mCherry (AAV2-retro) expression in ECT. Scale bar = 100 μm. d EGFP (AAV11) and mCherry (AAV2-retro) expression in VMH. Scale bar = 100 μm. e Quantification of AAV11- and AAV2-retro-infected projection neurons. Statistical values are presented as mean ± SEM (n = 4/group). Statistical analyses were performed through unpaired two-tailed Student’s t tests, with significant differences being expressed by the p value. ****p < 0.0001. Source data are provided as a Source Data file.

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