Fig. 5: AAV11 can be used for analyzing functional networks.
a Schematic diagram of AAVs injections and fiber implantations to monitor neural activities of NAc-projecting VTA neurons with GCaMP6m. AAV11-hSyn-Cre and AAV9-DIO-GCaMP6m (2 × 109 VG each virus) were injected into NAc and VTA, respectively. An optical fiber was implanted into VTA for the detection of calcium flux. Diagram was created with BioRender.com. b Schematic diagram of calcium transient monitoring in mice under the reward behavior tasks. c The GCaMP6m fluorescence in VTA. Scale bar = 200 μm. d Average ΔF/F signals were recorded using dual-viral vector strategy during the behavior tasks. Data were presented as mean ± SEM (n = 4/group). e A representative trace of GCaMP6m related ΔF/F signals. Black arrows indicate when the animal licked the sucrose solution. f Schematic diagram of AAV11-hSyn-GCaMP6s injection and fiber implantations. 100 nL of AAV11-hSyn-GCaMP6s (2.72 × 1013 VG/ml) were injected into NAc. Diagram was created with BioRender.com. g Average ΔF/F signals were recorded using single-viral vector strategy during the behavior tasks. Data are presented as mean ± SEM (n = 4/group). Source data are provided as a Source Data file.
