Fig. 7: Comparison of circuit connections between Alzheimer’s disease and wild-type mice using AAV11 tracing. | Nature Communications

Fig. 7: Comparison of circuit connections between Alzheimer’s disease and wild-type mice using AAV11 tracing.

From: AAV11 enables efficient retrograde targeting of projection neurons and enhances astrocyte-directed transduction

Fig. 7: Comparison of circuit connections between Alzheimer’s disease and wild-type mice using AAV11 tracing.

a Schematic diagram of virus injection. AAV11-EF1α-mCherry (6 × 109 VG per mouse) and CTB-488 (cholera toxin subunit B binding fluorescein 488, used to indicate the injection site; Thermo Fisher Scientific) were mixed (a volume ratio of 5:1, 200 nL per mouse) and injected into the dorsal hippocampus (dHPC) area of APP/PS1 (AD) transgenic mice and wild-type (WT) mice (C57BL/6) respectively. Diagram was created with BioRender.com. b The fluorescence distribution of mCherry at the injection site of dHPC. Scale bar = 1 mm. c Representative images of labeled neurons in upstream areas of dHPC, 3 weeks after AAVs injection. Scale bar = 200 μm. d Quantitative of mCherry-labeled projection neurons in upstream areas. Cont-dHPC contralateral dHPC, ENT entorhinal cortex, MSC medial septal complex. Statistical values are presented as mean ± SEM (n = 3/group). Statistical analyses were performed through unpaired two-tailed Student’s t tests, with significant differences being expressed by the p value. *p < 0.05,**p < 0.01. Source data are provided as a Source Data file.

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