Fig. 3: Anti-V. dahliae activity of BHA relies on its inhibition of VdPDA1.
From: Inhibition of chitin deacetylases to attenuate plant fungal diseases
a Scheme of the anti-virulence analysis methodology. b Disease phenotypes and vascular discoloration of the stems and tissues of cotton seedlings. ddH2O was used as the solvent for BHA. c Fungal biomass, as determined by qRT-PCR, on cotton plants infected by wild-type VdPDA1 with BHA pre-treatment. d Scheme of the root-dip methodology. e, f Pre-treatment of cotton with BHA restores the expression of GhMPK6 and GhRbohD that was suppressed by VdPAD1. The expression patterns of GhMPK6 (e) and GhRbohD (f) in cotton roots at 36 hpi with the wild-type (WT), the knockout mutant (VdΔpda1), and the complementary transformants (VdΔpda1/cPDA1D55A, H201A, VdΔpda1/cPDA1) were determined by RT-qPCR using cDNA as a template. Three independent replicates were used for measurement, and each experiment consisted of 3 cotton samples per V. dahliae infection. Bars in c, e, and f represent mean values, whiskers represent mean ± SEM, and black circles represent each replicate value (n = 3). Statistical significance was determined by Student’s two-sided unpaired t test. Source data are provided as a Source Data file.
