Fig. 6: Uhrf1 is essential for tumor growth in a mouse model of Kras-driven lung cancer.

a, b H&E images of representative tumor-bearing lungs from UKP and KP mice (a) and UK and K mice (b). c Quantification of tumor burden from the (U)KP (left) and (U)K (right) cohort. Horizontal lines represent mean values with one standard deviation error bars. Significance is calculated using two-sided unpaired Student’s t test; **p = 0.0061, ***p = 0.0003. The experiment has been performed twice in (U)KP and once in (U)K mice. d Survival analysis of KP (n = 18), U + /-KP (n = 14) and UKP mice (n = 17). Day 0 denotes the day of AdCre administration. Significance calculated using log-rang test, p = 3.4454e-07. e Immunohistochemistry for Uhrf1 expression in lung sections from representative KP (top) and UKP (bottom) mice. Arrows point to examples of Uhrf1-positive nuclei. Representative images of n = 7 (KP) and n = 16 (UKP) animals. f EdU and Uhrf1 co-immunostaining of a KP mouse tumor 11 weeks post Cre treated with EdU. Arrows point to a double-positive nucleus. g Quantification of Uhrf1 and EdU double-positive cells from EdU treated mice. Number of individual images quantified: n = 15 (KP, UKP), n = 5 (control no-virus mouse). Bars represent means with standard deviation error bars; *p = 0.031, **p = 0.0086, ns - not significant by Kruskal–Wallis test. h Western blot for UHRF1 protein expression in UKP mouse lung cancer cell line infected with the indicated adenovirus and control A549 cells treated with the indicated siRNAs. AdEmpty – empty adenovirus, AdCre – Cre-expressing adenovirus. The experiment was repeated twice. Source data are provided as a Source data file.