Fig. 2: Suppression of SUMOylation attenuates OVA- or IL-13-induced goblet cell metaplasia.
From: SUMOylation of Rho-associated protein kinase 2 induces goblet cell metaplasia in allergic airways
a OVA-sensitized mice were atomized with 1% OVA or an equal volume of NS for 30 min, once daily for 7 d. 2 h after each OVA atomization, mice were intratracheally administered with either 50 μl/mouse of 2-D08 at the dosages of 10 and 30 mg/kg or vehicle. Scale bar, 10 μm or 5 μm. b–f 24 h after the last OVA challenge, mice were subjected to examination of methacholine-provoked airway hyperreactivity (f, n = 6, P values: 0.0002, 0.0003, <0.0001, <0.0001, <0.0001, <0.0001; 0.0221, 0.0046, 0.0018, 0.0348, 0.025; 0.0029, 0.0002, 0.0001, 0.0004, <0.0001). BALFs were prepared for cell counting and classification (c, n = 6, P values: <0.0001, <0.0001, <0.0001, <0.0001, <0.0001; 0.0112, 0.0424, 0.0098; <0.0001, 0.0155, <0.0001), and lungs were harvested for paraffin-embedded sectioning for H&E staining (b, scale bar, 10 μm or 5 μm), PAS staining and immunostaining of Muc5AC (d, scale bar, 10 μm), their semi-quantification. (e, n = 6, P values: <0.0001, <0.0001, <0.0001; <0.0001, <0.0001, <0.0001). g Mice were intratracheally instilled with IL-13 (12 μg/mouse) to induce airway goblet cell metaplasia, following intratracheal instillation of 2-D08 at the dosages of 10 and 30 mg/kg. h, i Lungs were subjected to paraffin-embedded sectioning for PAS and Muc5AC staining (h, scale bar, 10 μm) and their semi-quantification (i, n = 6, P values: <0.0001, <0.0001, <0.0001; <0.0001, <0.0001, <0.0001). Mean ± SD, One-way ANOVA and Tukey-Kramer multiple comparisons test, *, +P < 0.05, **, ++P < 0.01. Source data are provided as a Source Data file.
