Fig. 3: Suppression of SUMOylation inactivates ROCK2 in response to OVA or IL-13 stimulation in bronchial epithelia or 16HBE cells.
From: SUMOylation of Rho-associated protein kinase 2 induces goblet cell metaplasia in allergic airways
a Quantitative phosphoproteomic analysis in 16HBE cells treated with IL-13 (100 ng/ml) at 6 h post and during pretreatment with 2-D08 (30 μM). The bar graph shows the quantitative results of phosphorylated sites. b Enrichment and analysis of the protein domains of differentially expressed phosphorylated peptides. c The altered phosphorylation levels on the key phosphorylation sites of ROCK2 upon IL-13 or/and 2-D08 treatment. d, e Western analyses in 16HBE cells treated with IL-13 at 6 h post and during treatment with 2-D08 (n = 3, P values: 0.0002, 0.0015, 0.0016; <0.0001, 0.0076, 0.0003). f–m Lungs and bronchi from Fig. 2g and Fig. 2a were subjected to immunofluorescence staining and western analyses, respectively, each n = 6 or 3, P values: <0.0001, <0.0001, <0.0001 (g); 0.0015, 0.009, 0.0023 (i); <0.0001, <0.0001, <0.0001 (k); 0.0022, 0.0364, 0.0089 (m). Scale bar, 5 μm. Mean ± SD, One-way ANOVA and Tukey-Kramer multiple comparisons test, +P < 0.05, ++, **P < 0.01. Source data are provided as a Source Data file.
