Fig. 3: Wtap deficiency impairs mast cell proliferation and FcεRI expression.
a Wtapfl/fl bone marrow cells were transduced with a lentiviral vector expressing a GFP-Cre fusion protein, following by sorting of the GFP+ cells and intracellular staining to verify Wtap protein depletion. Left, one representative staining for Wtap expression. Right, Wtap mean fluorescence intensity (MFI) in N = 7 independent experiments (ratio compared to control samples). Mean ± SD. Paired t test, two-tailed. b Expression of Mettl3 measured by intracellular staining in cells as in (a). N = 4 independent experiments. Mean ± SD. Paired t test, two-tailed. c Proliferation (measured by BrdU incorporation) of cells as in (a). Left, one representative experiment. Right, results of N = 3 independent experiments (ratio compared to control samples). Mean ± SEM. Paired t test, two-tailed. d Expression of surface FcεRI in cells lacking Wtap. Left, one representative experiment. Right, results of N = 3 independent experiments. Mean ± SEM. Paired t test, two-tailed. Source data are provided as a Source data File.
