Fig. 2: LC-OzID-MS(/MS) data for selected fatty acids in human plasma.
From: Ozone-enabled fatty acid discovery reveals unexpected diversity in the human lipidome
Data obtained by DIA UPLC-OzID-MS (a and c) and the associated DDA UPLC-OzID-MS/MS acquisition (b and d) from AMPP derivatized fatty acids from hydrolyzed human plasma (NIST 1950 SRM). Shown are extracted ion chromatograms and (tandem) mass spectra consistent with identification of FA 18:1n-9 (oleic acid), FA 18:1n−2, FA 18:1n−3, FA 20:3n−6,9,12 (dihomo-γ-linolenic acid), FA 20:3n−6,9,15 (sciadonic acid), and FA 20:3n−9,12,15 (Mead acid). The extracted ion chromatograms of the data-dependent acquisition contain only a few points across each chromatographic peak, as multiple precursors are mass selected consecutively according to a highly segmented target list. Source data are provided as a Source Data file.
