Fig. 6: Mutation of C8 reduces dimer formation under non-reducing conditions. | Nature Communications

Fig. 6: Mutation of C8 reduces dimer formation under non-reducing conditions.

From: Structural and functional fine mapping of cysteines in mammalian glutaredoxin reveal their differential oxidation susceptibility

Fig. 6: Mutation of C8 reduces dimer formation under non-reducing conditions.

A 200-ns MD simulation of three GLRX monomers in solution. Each monomer is shown in different color and cysteines involved in asymmetric and symmetric interfaces are shown as yellow spheres. See supplementary Movies 1 and 2 for the corresponding trajectories. Reducing (B) and non-reducing (C) gels of GLRX mutants stained with Coomassie Blue. 10 μg of protein was loaded per lane. Gel is representative of n = 3 independent experiments.

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