Table 5 Detailed statistics for Fig. 5
From: Amphetamine disrupts dopamine axon growth in adolescence by a sex-specific mechanism in mice
Statistical test | Factor | n | Statistic | 95% confidence interval | p value (adjusted where appropriate) | Corresponding figure | |
|---|---|---|---|---|---|---|---|
A | 4/group | Fig. 5d | |||||
One-way ANOVA | sgRNA | F (4, 15) = 3.857 | 0.0239 | ||||
Dunnett’s multiple comparisons test | LacZ vs. Dcc 1 | q(15) = 3.54 | −1.649 to −0.2137 | 0.0101 | |||
Dunnett’s multiple comparisons test | LacZ vs. Dcc 2 | q(15) = 2.68 | −1.423 to 0.01245 | 0.0547 | |||
Dunnett’s multiple comparisons test | LacZ vs. Dcc 3 | q(15) = 1.514 | −1.116 to 0.3193 | 0.3928 | |||
Dunnett’s multiple comparisons test | LacZ vs. Dcc 4 | q(15) = 2.842 | −1.465 to −0.03021 | 0.0401 | |||
B | 4/group | Fig. 5e | |||||
One-way ANOVA | sgRNA multiplexes | F (4, 15) = 5.618 | 0.0057 | ||||
Dunnett’s multiple comparisons test | LacZ vs. Dcc 1, 2 | q(15) = 0.2666 | −2.353 to 1.934 | 0.9963 | |||
Dunnett’s multiple comparisons test | LacZ vs. Dcc 1,3 | q(15) = 3.251 | −4.699 to −0.4115 | 0.018 | |||
Dunnett’s multiple comparisons test | LacZ vs. Dcc 1,4 | q(15) = 1.104 | −3.012 to 1.276 | 0.6472 | |||
Dunnett’s multiple comparisons test | LacZ vs. Dcc 1,2,3,4 | q(15) = 3.584 | −4.96 to −0.6729 | 0.0093 | |||
C | LacZ = 4 Dcc = 5 | Fig. 5i | |||||
One-tailed Unpaired t test | sgRNA multiplexes | t(7) = 2.140 | −0.01661 to 0.3338 | 0.0348 | |||
D | LacZ = 3 Dcc = 3 | Fig. 5j | |||||
Two-tailed Unpaired t test | sgRNA multiplexes | t(4) = 5.028 | 0.1294 to 0.4486 | 0.0073 | |||
E | LacZ = 3 Dcc = 3 | Fig. 5k | |||||
Pearson r | r = 0.9251, r2 = 0.8558 | 0.4554 to 0.9919 | 0.0082 | ||||
F | LacZ + Saline = 9 LacZ + AMPH = 5 Dcc + Saline = 6 Dcc + AMPH = 7 | Fig. 5m | |||||
Generalized Estimating Equations analysis (GEE) | Drug (between subjects) | Wald Chi-Square = 4.3 (df = 1) | <0.001 | ||||
Construct (between subjects) | Wald Chi-Square = 0.322 (df = 1) | 0.38 | |||||
Session (within subjects) | Wald Chi-Square = 158.053 (df = 13) | <0.001 | |||||
Drug × Construct | Wald Chi-Square = 5.577 (df = 1) | 0.018 | |||||
Construct × Session | Wald Chi-Square = 25.497 (df = 13) | 0.02 | |||||
Drug × Session | Wald Chi-Square = 37.392 (df = 13) | <0.001 | |||||
Drug × Construct × Session | Wald Chi-Square = 35.645 (df = 13) | <0.001 | |||||
G | LacZ + Saline = 9 LacZ + AMPH = 5 Dcc + Saline = 6 Dcc + AMPH = 7 | Fig. 5m (inset) | |||||
Two-way ANOVA | Interaction | F (1, 25) = 8.37 | 0.0078 | ||||
sgRNA construct (between subject) | F (1, 25) = 0.3124 | 0.5812 | |||||
Drug (between subject) | F (1, 25) = 4.317 | 0.0482 | |||||
Tukey’s multiple comparisons test | LacZ:Sal vs. LacZ: Amph | q(25) = 4.836 | −6.18 to −0.6699 | 0.0109 | |||
Tukey’s multiple comparisons test | LacZ:Sal vs. Dcc:Sal | q(25) = 3.554 | −4.982 to 0.2248 | 0.0822 | |||
Tukey’s multiple comparisons test | LacZ:Sal vs. Dcc: Amph | q(25) = 3.035 | −4.145 to 0.5118 | 0.1664 | |||
Tukey’s multiple comparisons test | LacZ: Amph vs. Dcc:Sal | q(25) = 1.361 | −1.945 to 4.037 | 0.7717 | |||
Tukey’s multiple comparisons test | LacZ: Amph vs. Dcc: Amph | q(25) = 2.271 | −1.147 to 4.363 | 0.3937 | |||
Tukey’s multiple comparisons test | Dcc:Sal vs. Dcc: Amph | q(25) = 0.8395 | −2.041 to 3.165 | 0.9331 | |||
H | LacZ + Saline = 9 LacZ + AMPH = 5 Dcc + Saline = 7 Dcc + AMPH = 10 | Fig. 5o | |||||
Two-way ANOVA | Interaction | F (1, 27) = 1.069 | 0.3103 | ||||
sgRNA construct | F (1, 27) = 0.8237 | 0.3721 | |||||
Drug | F (1, 27) = 0.043 | 0.8373 | |||||
I | LacZ + Saline = 9 LacZ + AMPH = 5 Dcc + Saline = 7 Dcc + AMPH = 10 | Fig. 5p | |||||
Two-way ANOVA | Interaction | F (1, 27) = 17.94 | 0.0002 | ||||
sgRNA construct | F (1, 27) = 0.2923 | 0.5932 | |||||
Drug | F (1, 27) = 7.713 | 0.0098 | |||||
Sidak’s multiple comparisons test | LacZ:Sal vs. LacZ: Amph | q(27) = 0.0004 | −9.499 to −2.326 | 0.0004 | |||
Sidak’s multiple comparisons test | LacZ:Sal vs. Dcc:Sal | q(27) = 0.0509 | −6.355 to 0.1249 | 0.0645 | |||
Sidak’s multiple comparisons test | LacZ:Sal vs. Dcc: Amph | q(27) = 0.29 | −4.84 to 1.068 | 0.3984 | |||
Sidak’s multiple comparisons test | LacZ: Amph vs. Dcc:Sal | q(27) = 0.1759 | −0.9674 to 6.562 | 0.2384 | |||
Sidak’s multiple comparisons test | LacZ: Amph vs. Dcc: Amph | q(27) = 0.0155 | 0.5053 to 7.548 | 0.0186 | |||
Sidak’s multiple comparisons test | Dcc:Sal vs. Dcc: Amph | q(27) = 0.6918 | −1.939 to 4.398 | 0.8612 | |||
J | LacZ + Saline = 9 LacZ + AMPH = 5 Dcc + Saline = 7 Dcc + AMPH = 10 | Fig. 5q | |||||
Two-way ANOVA | Interaction | F (1, 25) = 2.973 | 0.097 | ||||
sgRNA construct | F (1, 25) = 3.006 | 0.0953 | |||||
Drug | F (1, 25) = 18.44 | 0.0002 |
