Fig. 3: Lipids mediate BceB-BceS interaction.
From: Architecture of a complete Bce-type antimicrobial peptide resistance module
a Two rotated views of the atomic model of the BceAB-S complex. Individual protein chains and TM helices are colored the same as in Fig. 1. Purple sticks with surrounding transparent purple cryo-EM density indicate lipid molecules identified at the interface between BceB and BceS. Blue dashed lines indicate the approximate boundary of the lipid bilayer. b View from the extracellular space of the TM helix arrangement in the BceAB-S complex. Individual TM helices are labeled according to their number and whether they belong to BceB or BceS. Purple sticks with surrounding transparent purple cryo-EM density indicate lipid molecules that mediate interaction between BceB and BceS. The previously identified UPP binding pocket is indicated with a black arrow and is located on the opposite side of BceB as the site of BceS interaction. c, d Zoomed in view of lipid densities observed between BceB and BceS. Lipids are shown as magenta ball-and-stick with surrounding magenta mesh cryo-EM map. e Zoomed in view of the protein-protein interaction between BceB-TM3 and BceS.
