Fig. 1: H1.2 is enriched in beige/brown adipocytes and senses temperature change.
From: Linker histone variant H1.2 is a brake on white adipose tissue browning
10-week-old male mice were used in a–d and g–l; while 14-week-old male mice were used in e and f. a Representative mouse tissue expression profile of H1.2 (pooled samples from two individual mice for each lane; repeated three times independently with similar results obtained). b Relative H1.2 mRNA level in different mouse fat pads (n = 4 per group; one-way ANOVA with Tukey’s multiple comparisons test). PH1.2 (eWAT vs iWAT) < 0.0001, PH1.2 (eWAT vs BAT) < 0.0001, PH1.2 (iWAT vs BAT) < 0.0001. c, d H1.2 and Ucp1 mRNA (c) and protein (d) levels in mouse iWAT and BAT at 22 °C or for 3 days at 6 °C (n = 4 per group in c; n = 3 per group in d; unpaired two-tailed Student’s t test. e H1.2 and Ucp1 mRNA levels in mouse iWAT and BAT at 22 °C or for 4-week at 30 °C (22 °C group, n = 5; 30 °C group, n = 6; unpaired two-tailed Student’s t test). f H1.2 and Ucp1 protein levels in mouse iWAT and BAT at 22 °C or for 4-week at 30 °C (n = 3 per group). g Relative H1.2 mRNA level in stromal vascular fraction (SVF) and mature adipocytes (MA) (n = 3; unpaired two-tailed Student’s t test; repeated three times independently with similar results). h Representative H1.2 protein level in SVF and MA (pooled samples from three individual mice for each lane; repeated three times independently with similar results obtained). i, j mRNA (i) and protein (j) levels of H1.2 during primary beige adipocytes differentiation at indicated time (n = 3 per group; unpaired two-tailed Student’s t-test). k, l Representative H1.2 mRNA (k) and protein (l) level during primary brown adipocytes differentiation at indicated time (n = 3 per time in k; n = 2 per time in l; unpaired two-tailed Student’s t test; repeated three times independently with similar results obtained). Data are mean ± S.D. *P < 0.05, **P < 0.01. Source data and exact P values are provided in a Source data file.
