Fig. 10: Overexpression of Il10rα abolishes the metabolic improvement of H1.2AKO mice.

a Experimental design. Normal chow (NC)-fed WT and H1.2AKO mice were injected with AAV-Vehicle or AAV-Il10rα at 10-week-old, and maintained on NC-fed conditions until 25-week-old. b Il10rα level in iWAT of AAV-Vehicle or AAV- Il10rα injected NC-fed WT and H1.2AKO mice. c–e Body weight (c), fat mass (d), and tissue weights (e) of AAV-Vehicle or AAV-Il10rα injected NC-fed WT and H1.2AKO mice. f–h Blood glucose (f), glucose tolerance test (g), insulin tolerance test (h) of AAV-Vehicle or AAV-Il10rα injected NC-fed WT and H1.2AKO mice. i, j Oxygen consumption (i) and energy expenditure (EE) (j) of AAV-Vehicle or AAV-Il10rα injected NC-fed WT and H1.2AKO mice. k Representative H&E staining of eWAT/iWAT of WT and H1.2AKO mice. Scale bar = 50 μm. l qPCR of genes related to thermogenesis and lipid β-oxidation in iWAT of AAV-Vehicle or AAV-Il10rα injected NC-fed WT and H1.2AKO mice. For panels a–l, n = 5 for AAV-Vehicle injected WT mice, n = 4 for AAV-Vehicle or AAV-Il10rα injected H1.2AKO mice. Two-tailed ANCOVA with body weight as a covariate was used in i and j; otherwise, unpaired two-tailed Student’s t test was used for comparing two indicated groups. Data are mean ± S.D. *P < 0.05, **P < 0.01, AAV-Vehicle injected H1.2AKO mice compared with AAV-Vehicle injected WT mice; ^#P < 0.05, ^##P < 0.01, AAV-Il10rα injected H1.2AKO mice compared with AAV-Vehicle injected H1.2AKO mice. Source data and exact P values are provided in a Source data file. m Summary of our findings. In WT beige adipocytes, H1.2 binds to Il10rα, thus upregulating Il10rα transcription. Increased Il10rα reduces iWAT browning. While in H1.2 deletion beige adipocytes, reduced Il10rα level and increased browning are observed.