Fig. 3: H1.2 regulates iWAT browning under cold exposure.

In all, 10–11 weeks-old male mice were used in this figure. a Rectal temperature of WT and H1.2AKO mice before and after cold exposure (6 °C for 3 days; WT mice, n = 5; H1.2AKO mice, n = 3; unpaired two-tailed Student’s t test). b, c Oxygen consumption (b) and energy expenditure (EE) (c) with quantitative results of WT and H1.2AKO mice upon cold exposure (n = 6 per group; two-tailed ANCOVA with body weight as a covariate). d Representative images of H&E and Ucp1 staining of iWAT upon cold exposure (n = 6 per group). Scale bar = 50 μm. e qPCR analysis of thermogenic genes and lipid β-oxidation genes in iWAT of WT and H1.2AKO mice upon cold exposure (WT mice, n = 5; H1.2AKO mice, n = 3; unpaired two-tailed Student’s t test). f H1.2 mRNA level in iWAT injected with AAV-Vehicle (control) or AAV-H1.2 (H1.2OE) after cold exposure (n = 6 per group; unpaired two-tailed Student’s t test). g H1.2 and Ucp1 protein levels in iWAT of control and H1.2OE mice upon cold exposure (n = 3 per group). h Rectal temperature of control and H1.2OE mice before and after cold exposure (n = 6 per group; unpaired two-tailed Student’s t test). i, j Representative images of H&E (i) and Ucp1 staining (j) in iWAT of control and H1.2OE mice after cold exposure (n = 6 per group). Scale bar = 50 μm. k qPCR analysis of thermogenic genes in iWAT of control and H1.2OE mice after cold exposure (n = 6 per group; unpaired two-tailed Student’s t test). Data are mean ± S.D. *P < 0.05, **P < 0.01. Source data and exact P values are provided in a Source data file.