Fig. 4: Il10rα is downregulated in mature adipocytes of H1.2AKO mice.
From: Linker histone variant H1.2 is a brake on white adipose tissue browning
10-week-old male mice were used in this figure. a Enrichment of differentially expressed genes in iWAT of H1.2AKO mice (two samples for each group, each sample was a pool of two individual mice). b, c qPCR analysis of genes related to humoral immune pathways (b), and Il10rα or Il10rβ (c) in iWAT of WT and H1.2AKO mice (WT mice, n = 4; H1.2AKO mice, n = 3). d, e Il10rα protein level (d) and representative immunofluorescence staining of Il10rα (red) and Perilipin 1 (green) (e) in iWAT of WT and H1.2AKO mice (n = 3 per group). Scale bar = 20 μm. f Representative images of colocalized Il10rα (red) and Perilipin 1 (green) in iWAT of WT and H1.2AKO mice; magnified orthogonal sectioning view of regions in the insert boxes (n = 3 per group). Scale bar = 10 μm. g, h H1.2 and Il10rα mRNA (g) and protein levels (h) in stromal vascular fraction (SVF) and mature adipocytes (MA) fractionated from iWAT of WT and H1.2AKO mice (n = 3 per group). In g: PH1.2/Il10rα (WT SVF vs WT MA) < 0.0001, PH1.2/Il10rα (H1.2AKO SVF vs WT MA) < 0.0001, PH1.2/Il10rα (WT MA vs H1.2AKO MA) < 0.0001. i Il10rα mRNA level in iWAT of WT and H1.2AKO mice after cold exposure (WT mice, n = 5; H1.2AKO mice, n = 3). j Il10rα protein level in iWAT of WT and H1.2AKO mice after cold exposure (n = 3 per group). k, l H1.2 and Il10rα mRNA (k) and protein (l) levels in SVF and MA fractionated from iWAT of WT and H1.2AKO mice after cold exposure (n = 3 per group). In k: PH1.2 (WT SVF vs WT MA) < 0.0001, PH1.2 (H1.2AKO SVF vs WT MA) < 0.0001, PH1.2 (WT MA vs H1.2AKO MA) < 0.0001; PIl10rα (WT SVF vs WT MA) = 0.0002, PIl10rα (H1.2AKO SVF vs WT MA) = 0.0002, PIl10rα (WT MA vs H1.2AKO MA) = 0.0026. Data are mean ± S.D. Unpaired two-tailed Student’s t test in b, c and i; one-way ANOVA with Tukey’s multiple comparisons test in g and k. *P < 0.05, **P < 0.01. Source data and exact P values are provided in a Source data file.
