Fig. 6: H1.2 regulates cold-induced iWAT browning through Il10rα.
From: Linker histone variant H1.2 is a brake on white adipose tissue browning
a Experimental design. WT and H1.2AKO mice were injected with AAV-Il10rα (left iWAT pad) and AAV-Vehicle (right iWAT pad) at 8-week-old, 3 weeks later, then exposed under 6 °C for 3 days. b Il10rα mRNA level in iWAT after cold exposure (6 °C for 3 days) in the indicated groups (n = 5 per group; one-way ANOVA with Tukey’s multiple comparisons test). PIl10ra (WT+AAV-Vehicle vs H1.2AKO+AAV-Vehicle) < 0.0001, PIl10ra (WT+AAV-Vehicle vs WT+AAV-Il10ra) < 0.0001, PIl10ra (WT+AAV-Il10rα vs H1.2AKO+AAV-Il10rα) = 0.6088. c Representative Il10rα staining of iWAT injected with AAV-Il10rα or AAV-Vehicle after cold exposure (n = 5 per group). Scale bar = 50 μm. d, e Representative H&E (d) and Ucp1 staining (e) of iWAT in WT and H1.2AKO mice injected with AAV-Il10rα or AAV-Vehicle after cold exposure (n = 5 per group). Scale bar = 50 μm. f mRNA levels of thermogenic genes in iWAT of WT and H1.2AKO mice injected with AAV-Il10rα or AAV-Vehicle after cold exposure (n = 5 per group; one-way ANOVA with Tukey’s multiple comparisons test). PUcp1 (WT+AAV-Vehicle vs H1.2AKO+AAV-Vehicle) = 0.0019, PUcp1 (WT+AAV-Il10rα vs H1.2AKO+AAV-Il10rα) > 0.9999, PDio2 (WT+AAV-Vehicle vs H1.2AKO+AAV-Vehicle) < 0.0001, PDio2 (WT+AAV-Il10rα vs H1.2AKO+AAV-Il10rα) = 0.9264, PCidea (WT+AAV-Vehicle vs H1.2AKO+AAV-Vehicle) < 0.0001, PCidea (WT+AAV-Il10rα vs H1.2AKO+AAV-Il10rα) = 0.6903, PCidec (WT+AAV-Vehicle vs H1.2AKO+AAV-Vehicle) = 0.0833, PCidec (WT+AAV-Il10rα vs H1.2AKO+AAV-Il10rα) = 0.8226, PCox8b (WT+AAV-Vehicle vs H1.2AKO+AAV-Vehicle) < 0.0001, PCidea (WT+AAV-Il10rα vs H1.2AKO+AAV-Il10rα) = 0.9988. g Representative H1.2, Ucp1 and Il10rα protein levels in iWAT of WT and H1.2AKO mice injected with AAV-Il10rα or AAV-Vehicle after cold exposure (n = 3 per group). Data are mean ± S.D. **P < 0.01; ns not significant. Source data and exact P values are provided in a Source data file.
