Fig. 7: H1.2AKO mice have a better metabolic status after long-term normal chow-feeding.
From: Linker histone variant H1.2 is a brake on white adipose tissue browning
a Body weight of normal chow (NC)-fed WT and H1.2AKO mice at 18-week-old and 28-week-old (WT mice, n = 4; H1.2AKO mice, n = 5; unpaired two-tailed Student’s t test). b, c Fat mass or lean mass (b) and different tissue weights (c) of 28-week-old NC-fed WT and H1.2AKO mice (WT mice, n = 4; H1.2AKO mice, n = 5; unpaired two-tailed Student’s t test). d Representative H&E staining of eWAT/iWAT/BAT of 28-week-old NC-fed WT and H1.2AKO mice (WT mice, n = 4; H1.2AKO mice, n = 5). Scale bar = 50 μm. e Average adipocyte area of eWAT and iWAT of 28-week-old NC-fed WT and H1.2AKO mice (n = 3 per group; unpaired two-tailed Student’s t test). f, g Oxygen consumption (f) and energy expenditure (EE) (g) of 28-week-old NC-fed WT and H1.2AKO mice (WT mice, n = 4; H1.2AKO mice, n = 5; two-tailed ANCOVA with body weight as a covariate). h, i glucose tolerance test (h) and insulin tolerance test (i) of 28-week-old NC-fed WT and H1.2AKO mice (WT mice, n = 4; H1.2AKO mice, n = 5; unpaired two-tailed Student’s t test). j qPCR of genes associated with lipogenesis, lipid β-oxidation and thermogenesis in iWAT of 28-week-old NC-fed WT and H1.2AKO mice (WT mice, n = 4; H1.2AKO mice, n = 5; unpaired two-tailed Student’s t test). k H1.2, Ucp1, and Il10rα levels in iWAT of 28-week-old NC-fed WT and H1.2AKO mice (WT mice, n = 4; H1.2AKO mice, n = 3). Data are mean ± S.D. *P < 0.05, **P < 0.01. Source data and exact P values are provided in a Source data file.
