Fig. 9: H1.2AKO mice are resistant to HFD-induced obesity.

a, b mRNA (a) and protein (b) levels of H1.2 in iWAT and BAT of normal chow (NC)-fed or six-month HFD-fed WT mice (n = 4 per group; unpaired two-tailed Student’s t test). c H1.2 level in human subcutaneous adipose tissues (n = 5 for normal and n = 7 for obese; unpaired two-tailed Student’s t test). d, e qPCR of H1.2 in human subcutaneous adipose tissues (d) and correlation of H1.2 mRNA level with body mass index (BMI) (e) (n = 17 for normal and n = 12 for obese; unpaired two-tailed Student’s t test). f, g body weight (f), fat mass and lean mass (g) of 21-week HFD-fed WT and H1.2AKO mice (WT mice, n = 6; H1.2AKO mice, n = 5; unpaired two-tailed Student’s t test). h Oxygen consumption of HFD-fed WT and H1.2AKO mice (WT mice, n = 6; H1.2AKO mice, n = 5; two-tailed ANCOVA with body weight as a covariate). i Representative H&E staining of eWAT/iWAT/BAT of HFD-fed WT and H1.2AKO mice (WT mice, n = 6; H1.2AKO mice, n = 5). Scale bar = 50 μm. j, k glucose tolerance test (j) and insulin tolerance test (k) of HFD-fed WT and H1.2AKO mice (WT mice, n = 4; H1.2AKO mice, n = 5; unpaired two-tailed Student’s t test). l qPCR of genes associated with lipogenesis, lipid β-oxidation and thermogenesis in iWAT of HFD-fed WT and H1.2AKO mice (WT mice, n = 6; H1.2AKO mice, n = 5; unpaired two-tailed Student’s t test). m H1.2, Ucp1, and Il10rα protein levels in iWAT of HFD-fed WT and H1.2AKO mice (n = 3 per group). Data are mean ± S.D. *P < 0.05, **P < 0.01. Source data and exact P values are provided in a Source data file.