Fig. 3: Genome editing of the CsPDS gene of embryogenic protoplast cells of C. sinensis cv. Hamlin. | Nature Communications

Fig. 3: Genome editing of the CsPDS gene of embryogenic protoplast cells of C. sinensis cv. Hamlin.

From: Generation of the transgene-free canker-resistant Citrus sinensis using Cas12a/crRNA ribonucleoprotein in the T0 generation

Fig. 3

A Genome-edited embryos under regeneration. Er indicates ErCas12a. Lb indicates LbCas12aU. B Sanger sequencing confirmation of genome-edited lines in the CsPDS gene. TTTC in red: PAM (protospacer adjacent motif). Mutation indicates mutations in the edited embryos. The number of edited embryos for each genotype was also shown.

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