Fig. 6: Effect of mutation of CsLOB1 on ROS production and expression of cell wall related genes.

A Expression of orange.1t00600, Cs6g17190, Cs7g32410 and Cs9g17380, which are known to be up-regulated by CsLOB1 during Xcc infection, in the cslob1 mutant and wild-type C. sinensis cv. Hamlin with and without Xcc inoculation at 1 × 10⁷ cfu/mL with syringes. CsGAPDH, a housekeeping gene encoding glyceraldehyde-3-phosphate dehydrogenase in citrus was used as an endogenous control. Four biological replicates were used and mean values ± SD (standard deviation) (n = 4) are shown. Two-sided Student’s t test was used for statistical analysis, single asterisk (*) showed significant differences (P value of orange.1t00600, Cs6g17190, Cs7g32410 and Cs9g17380 are 0.0338, 0.000408, 0.000265 and 0.000325, respectively). Experiments were repeated at least two times with similar results and representative results are shown. B H₂O₂ concentration was quantified one day after inoculation. Four biological replicates were used for each experiment. Wild type and cslob1 mutant of C. sinensis leaves were inoculated with Xcc (1 × 10⁸ cfu/mL) or water using needleless syringes. Values represent means ± SD (n = 4). The experiment was repeated twice with similar results. All box plots include the median line, the box denotes the interquartile range (IQR), whiskers denote the rest of the data distribution and there are no outliers. The lower and upper hinges of the boxes correspond to the 25th and 75th percentiles. Source data are provided as a Source data file.