Fig. 5: Reduced SOM sound-evoked activity (non-recovery) after NIHL.
a Wide-field (WF) imaging experimental design for A1 SOMs. b Experimental setup. c Representative transcranial fluorescence responses of A1 SOMs from sham- and noise-exposed mice. d Average change in A1 SOM response threshold (orange) before and after noise-exposure. Note: Since we did not observe any sound-evoked activity in SOMs at 1 day after noise exposure, even at 80 dB SPL sounds, we did not assign a threshold shift at this time point, but it is >50 dB SPL. The average change in PN (gray) and PV (red) threshold reproduced from Fig. 4 (PV vs. PN vs. SOM: effect of cell-type, F = 31.9, p = 4.3 × 10−7). e Average sound-evoked responses of A1 SOMs. (Intensity vs. time, the effect of time, F  = 82.97, p < 1.1 × 10−10). f Average response gain of SOMs (orange) normalized to pre-noise-exposed gain at 1, 3, and 10 days after noise exposure. Normalized PN (gray) and PV (red) gain reproduced from Fig. 4. g Timetable of longitudinal 2P imaging. h Experimental setup illustrating 2P imaging. i Z-stack images of tracked SOMs. j Representative responses from a SOM before and after NIHL. k Average change in SOMs response threshold from noise (orange) and sham (gray) exposed mice (Noise vs. sham: effect of exposure, F = 16.60, p = 8.2 × 10−5). l Cumulative probability of SOM response threshold. Inset: Average mean threshold of SOMs per mouse. m Average responses of individual SOMs. n Average gain of individual SOMs normalized to pre-exposed gain from noise (orange) and sham (gray) mice. o Cumulative probability of SOMs gain. Inset: Average mean gain of SOMs per mouse. p Scatter plots of the gain of individual A1 L2/3 SOMs before and after NIHL. The dotted line represents unity. Insets: Bar graphs representing the number of neurons showing increased gain (↑ above unity) and reduced gain (↓ below unity) after NIHL. q Average change in response threshold and gain r of SOMs with Low-BF (left, n = 31 SOMs), Mid-BF (middle, n = 34 SOMs), and High-BF (right, n = 15 SOMs). *p < 0.05; statistical values are given in Supplementary Table 6.
