Fig. 1: Primary influenza infection induces strong early EFRs prior to GC formation. | Nature Communications

Fig. 1: Primary influenza infection induces strong early EFRs prior to GC formation.

From: Toll-like receptor mediated inflammation directs B cells towards protective antiviral extrafollicular responses

Fig. 1: Primary influenza infection induces strong early EFRs prior to GC formation.The alternative text for this image may have been generated using AI.

Shown are flow cytometric analyses of mediastinal lymph nodes (medLN) from C57BL/6 mice infected with influenza A/PR8 intra-nasally (i.n.) at seven days post-infection (dpi). a Identification of extrafollicular plasmablasts (EF PBs) by gating first on CD19lo/CD45Rlo then CD24+/CD38- and pre-GC/GC B cells on CD19+/CD45R+ then CD24hi/CD38lo. Presence of CD19lo/CD45Rlo and CD24+/CD38- populations at 5 dpi (left) and 7 dpi (right). b IRF8 and GL7 expression shown to confirm GC identity and IRF4 and CD138 expression shown to confirm EF PB identity (left), along with IgM and IgD expression of B cell subsets (right). c–e C57BL/6 mice (n = 3–4) were infected and medLN were collected on the days specified, measuring B cell frequencies of total cells (c), pre-GC/GC frequency of B cells (d), and EF frequency of B cells (e). Data in (c–e) represent mean ± 95% confidence interval (CI) of two independent experiments. Statistical significance determined by one-way ANOVA. ****p < 0.0001. Source data are provided as a Source Data file.

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