Fig. 2: EFRs generate influenza-specific antibody-secreting cells. | Nature Communications

Fig. 2: EFRs generate influenza-specific antibody-secreting cells.

From: Toll-like receptor mediated inflammation directs B cells towards protective antiviral extrafollicular responses

Fig. 2: EFRs generate influenza-specific antibody-secreting cells.The alternative text for this image may have been generated using AI.

a Influenza-specific ELISPOTS of sorted EF PBs and pooled non-EF cells from influenza-infected C57BL/6 mice (n = 6) for total Ig (left) and IgG2c (right). b Flow plots of HA-specific B cells identified using double HA-tetramer staining with subsequent phenotyping by additional markers as outlined in Fig. 1a. c–e Time course of HA-specific B cell subsets during influenza infection in C57BL/6 mice (n = 3–4), measuring frequency of HA-specific clones (c), HA-specific pre-GC/GC clones (d), and HA-specific EF PBs (e). Data in (a, c–e) represent mean ± 95% CI of two independent experiments. Statistical significance determined by two-tailed Student’s t-test with Welch’s correction or one-way ANOVA. *p < 0.05, **p < 0.01, ****p < 0.0001. Source data are provided as a Source Data file.

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