Fig. 4: Optimal EFR kinetics and protective antibodies require MyD88 and TRIF. | Nature Communications

Fig. 4: Optimal EFR kinetics and protective antibodies require MyD88 and TRIF.

From: Toll-like receptor mediated inflammation directs B cells towards protective antiviral extrafollicular responses

Fig. 4: Optimal EFR kinetics and protective antibodies require MyD88 and TRIF.The alternative text for this image may have been generated using AI.

Knockout and WT mice (n = 4–5) were infected with 10 PFU A/PR8 and medLNs were collected at 7 days post-infection (dpi). a Fold-difference of B cell subsets in TLR-deficient versus WT mice at 7 dpi. b–d Serum was transferred to C57BL/6 mice prior to infection with a lethal dose (100 PFU) of influenza A/PR8 the next day from naïve WT, DKO, and TKO mice (n = 4) (b) and influenza-infected, age/sex matched- WT and MyD88/TRIF-deficient (DKO) mice (n = 10) (c) or WT and TLR2/4/unc93b-deficient (TKO) mice (n = 10) (d) from 10 dpi. Shown is percent change in weight over the course of infection. Data in (a–d) represent mean ± 95% CI of two independent experiments. Statistical significance determined by two-way ANOVA and two-tailed Student’s t-test with Welch’s correction. **p < 0.01, ***p < 0.001, ****p < 0.0001, *****p < 0.00001 or indicated in subfigures. Source data are provided as a Source Data file.

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