Fig. 3: Cytokine induction in BALB/c mice given 7DW8-5 (2 µg; IN).

a Heatmap showing the differential expression of the genes (as indicated) in nasal turbinates (NT), which is an average of three independent experiments with each consisting of a group of 3 BALB/c mice 24 h after 7DW8-5 (2 µg) or saline administration by IN. The scale bar shows the average Z-score. b Induction of cytokines/chemokines in bronchoalveolar lavage (BAL), supernatants of homogenates of lung (LH) and nasal turbinates (NT), and sera 24 h after 7DW8-5 administration to mice (N = 6), as assessed using the Mouse Cytokine Array/Chemokine Array 31-Plex (MD31) platform (Eve Technologies). Cytokine concentrations are plotted (in circles) and averages shown (using bar graphs), and the fold-change seen with 7DW8-5 treatment is expressed at the top. c Shift toward IFN-\(\gamma\)-producing cells as presented by Uniform Manifold Approximation and Projection (UMAP) of total 27,532 single CD45+ cells from the lungs from 3 BALB/c mice 24 h after treating with 7DW8-5 or saline (also see Supplementary Fig. 5a). The UMAP was split into 15,397 single cells (7DW8-5, left) and 12,135 single cells (saline, right). Each cluster (as indicated) was identified by unsupervised clustering and colored separately. iNKT cells were identified based on the expression of the semi-invariant TCR Vα14-Jα18.