Fig. 1: The transcriptional landscape of single immune cells in tissue and blood from achalasia and controls. | Nature Communications

Fig. 1: The transcriptional landscape of single immune cells in tissue and blood from achalasia and controls.

From: A single-cell transcriptional landscape of immune cells shows disease-specific changes of T cell and macrophage populations in human achalasia

Fig. 1: The transcriptional landscape of single immune cells in tissue and blood from achalasia and controls.The alternative text for this image may have been generated using AI.

a A schematic representation of the experimental design and analysis with tissue sources, sorting strategy, and scRNA-seq results. Immune cells in tissue were dissociated from smooth muscle in the LES. The LES specimens were collected from patients with achalasia and benign esophageal leiomyoma (as controls) undergoing endoscopic procedure. After the leiomyomas were removed, the control tissue specimens were taken from the surrounding normal tissue 5 mm away from the leiomyoma without tumor invasion. b t-SNE plots of the major immune and non-immune cells found in LES tissue and blood. c, d Heatmap (c) and t-SNE plots (d) of the relative expressions of canonical marker genes for major immune and non-immune cells. e Major cluster proportions for LES tissue and blood between achalasia and controls, colored by cell type. f Major cluster proportions in LES tissue compared with blood. Source data are provided as a Source Data file.

Back to article page