Fig. 5: The MOM1 complex influences DNA methylation and chromatin accessibility at some endogenous RdDM sites.
From: The MOM1 complex recruits the RdDM machinery via MORC6 to establish de novo DNA methylation
a Boxplots and heatmaps showing the variation of CG, CHG, and CHH DNA methylation in phd1-2, aipp3-1, mom2-2, mom1-3, pial1/2, morc6-3 and morchex mutants vs Col-0 wild type over hypo CHH hcDMRs of the morchex mutant (n = 520). b the number and heatmap of overlapping of hypo CHH hcDMRs among aipp3-1, mom2-2, mom1-3, pial1/2, morc6-3 and morchex mutants over morchex mutant hypo CHH hcDMRs (n = 520). c Boxplot representing the expression level (RNA-seq signal normalized by RPKM) of the genomic bins of 1 kb from hypo CHH hcDMRs (n = 520) of the morchex mutant in Col-0, aipp3-1, phd1-2, pial1-2, pial2-1, mom2-2, mom1-3, pial1/2, morc6-3 and morchex mutants. d Metaplots and heatmaps representing ATAC-seq signal (mom1-3 minus Col-0), MOM1 ChIP-seq signal and Pol V ChIP-seq signal (subtracting control ChIP-seq signal) over regions with higher ATAC-seq signals in mom1-3 (n = 342) and shuffled regions. e Screenshots of ATAC-seq signals of Col-0 and mom1-3, ChIP-seq signals of MOM1-Myc and Pol V (subtracting control signal) as well as CG, CHG, and CHH DNA methylation level by WGBS over a representative RdDM site. In box plots of a and c, center line represents the median; box limits represent the 25th and 75th percentiles; whiskers represent the minimum and the maximum. Source data are provided as a Source Data file.
