Fig. 3: Proteomics profiling of individual biomolecular coronas. | Nature Communications

Fig. 3: Proteomics profiling of individual biomolecular coronas.

From: Multiomics analysis of naturally efficacious lipid nanoparticle coronas reveals high-density lipoprotein is necessary for their function

Fig. 3

a The comparison between lean/obese plasmas derived LNPcor entities. The mean intensity of each group was summarized from all identified corona proteins from each independent individual-plasma- (n = 8) and the pooled lean/obese plasma (n = 1) derived LNPcor. The uncomplexed LNPs and non-specifically bound lipid binding were evaluated, respectively. For box and whisker plots, each data point represents an individual LNPcor, averaged from duplicate measurements. The box represents the interquartile range (first quartile, median and third quartile). BG is the intensity summarized from background impurity controls. P value is obtained by two-tailed Mann-Whitney test. Source data are provided as a Source Data file. b The average ratio of major proteomic functional clusters summarized from lean and obese-individual-derived LNPcor. Source data are provided as a Source Data file. c The most abundant coronal proteins (RPA > 0.1%) in lean and plasma-derived LNPcor and the corresponding RPA in original plasmas (n = 2). RPA: relative protein abundance. CV: coefficient of variation. Source data are provided as a Source Data file. d An overview of major corona proteins in individual LNPcor (n = 2). UP, uncharacterized protein. e Hierarchical clustering of individual plasma derived LNPcor using proteomic fingerprints. Lean- and obese-plasma-derived LNPcor formed distinct clusters. Source data are provided as a Source Data file.

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