Fig. 5: Single-molecule photons detected before photobleaching.
From: Structural and photophysical characterization of the small ultra-red fluorescent protein
Small-molecule organic dyes, AlexaFluor647 and Cy5, were compared to smURFP+BV and TDsmURFP+BV. Single molecules doped in PVA were excited with a 638 nm laser at 76 or 264 W/cm2. a An average of 23,000 or 43,000 photons were detected before photobleaching from AlexaFluor647 when excited by 76 or 264 W/cm2 intensity, respectively. b An average of 59,000 or 47,000 photons were detected from Cy5. c-d An average of 100,000 and 120,000 or 92,000 and 94,000 photons were detected before photobleaching from (c) smURFP+BV or (d) TDsmURFP+BV, respectively. The average number of photons detected before photobleaching ± standard error of the mean (SEM) and the number of single molecules measured (n) is above each graph.
