Fig. 5: Loss of rlmN and treatment with menadione cause similar changes in the OG1RF proteome. | Nature Communications

Fig. 5: Loss of rlmN and treatment with menadione cause similar changes in the OG1RF proteome.

From: An RNA modification enzyme directly senses reactive oxygen species for translational regulation in Enterococcus faecalis

Fig. 5: Loss of rlmN and treatment with menadione cause similar changes in the OG1RF proteome.

Volcano plots showing changes in protein levels in OG1RF caused by a rlmN knockout (ΔrlmN) and b menadione treatment. P values were calculated using the two-sided Student’s t test. The log2 fold-change ( x axis) was plotted against the −log10( p value) ( y axis). A −log10( p value) of 1.30 threshold (P < 0.05) is denoted by the dotted horizontal line, while the vertical dotted lines represent fold-change values at ±1 SD. c Proteins detected in both menadione-treated and ΔrlmN without a P value cutoff. Pearson correlation R = 0.448, two-tailed p value < 0.0001. d Proteins that changed significantly (P < 0.05) in both menadione-treated and ΔrlmN cells. Cutoff was set at 1 SD, which is log2(fold-change) of ±0.639 for menadione treatment and log2(fold-change) of ±0.443 for ΔrlmN. Blue circles represent proteins that significantly up- or downregulated in both, relative to untreated, wild-type cells. These proteins are shown in Supplementary Table 4. Data shown are for three biological replicates. Red circles denote superoxide dismutase (SodA). Pearson correlation R = 0.900, two-tailed p value 0.0372.

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