Fig. 1: Syt1 is the main calcium sensor for fast axonal dopamine release.

A Generation of conditional knockout of Syt1 in DA neurons by crossing Syt1-floxed mice (Syt1^lox/lox) with DAT^IREScre mice. B Fast-scan cyclic voltammetry recording of Syt1 cKO^DA mice in the dorsal striatum. Representative traces (top) and quantification of peak amplitude (bottom) obtained with single-pulse electrical stimulation (1 ms, 400 µA) in Syt1^+/+ (n = 18 slices/9 mice), Syt^+/− (n = 16/8) and Syt1^−/− mice (n = 16/8). C Same, but in the ventral striatum (NAc core and shell, n = 18 slices/9 mice in Syt1^+/+, n = 16/8 in Syt^+/− and n = 16/8 in Syt1^−/−). D Representative traces (top) and quantification of peak amplitude (bottom) obtained in the VTA (n = 16 slices/9 mice in Syt1^+/+, n = 14/8 in Syt^+/− and n = 16/8 in Syt1^−/−) with aCSF containing nomifensine (DAT blocker) and sulpiride (D2 antagonist) (both at 5 µM), and pulse-train stimulation (30 pulses of 1 ms at 10 Hz, 400 µA). E Same for the SNc (n = 11 slices/6 mice in Syt1^+/+, n = 10/5 in Syt^+/− and n = 9/5 in Syt1^−/−). Error bars represent ± SEM and the statistical analysis was carried out by one-way ANOVAs followed by Tukey tests (ns, non-significant; *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001). Source data are provided as a Source Data file.