Fig. 5: Basal extracellular DA levels and total tissue DA are not altered in Syt1 cKODA mice. | Nature Communications

Fig. 5: Basal extracellular DA levels and total tissue DA are not altered in Syt1 cKODA mice.

From: Synaptotagmin-1-dependent phasic axonal dopamine release is dispensable for basic motor behaviors in mice

Fig. 5

A Schematic representation of the intracerebral microdialysis protocol performed on anesthetized Syt1 cKODA and control mice. B Quantification of total DA, DOPAC, serotonin and norepinephrine striatal content (ng/mg of total proteins) and DA/DOPAC ratio (n = 10 Syt1+/+ and 12 Syt1−/−). Statistical analysis was carried out by two-tailed unpaired t test. C Extracellular quantification (ng/mL) by microdialysis of the same molecules in the dorsal striatum (n (Syt1+/+/ Syt1−/− mice) = 10/12 for DA, 11/12 for DOPAC, 8/12 for 5-HT and 10/12 for NE) and in the mesencephalon (n (Syt1+/+/ Syt1−/− mice) = 9/12 for DA, 10/12 for DOPAC, 9/12 for 5-HT and 9/12 for NE) of Syt1 cKODA mice, with an average of three dialysates for each animal. Statistical analysis was carried out by two-way ANOVAs followed by Šidák’s tests. Error bars represent ± SEM (ns, non-significant; *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001). Source data are provided as a Source Data file.

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