Fig. 8: Adaptations of DA innervation in the striatum of Syt1 cKODA mice. | Nature Communications

Fig. 8: Adaptations of DA innervation in the striatum of Syt1 cKODA mice.

From: Synaptotagmin-1-dependent phasic axonal dopamine release is dispensable for basic motor behaviors in mice

Fig. 8

A Immunochemistry of striatal slices from 10 to 12-week-old Syt1+/+ and Syt1−/− mice (60x confocal) using (from top to bottom): tyrosine hydroxylase (TH), vesicular monoamine transporter 2 (VMAT2), dopamine transporter (DAT) and serotonin (5-HT) immunostainings. Scale bar = 50 µm. B Quantification of each signal surface (% of WT) in the dorsal and ventral striatum (n = 20 hemispheres/10 mice for both genotypes). C Same with signal intensity (n = 20 hemispheres/10 mice for both genotypes). D TH-immunoreactive cells in the midbrain of Syt1+/+ and Syt1−/− mice, identified by the brown DAB staining. E Stereological counts of DA neurons in the SNc (n = 5 Syt1+/+/5 Syt1−/−), VTA (n = 5/5), and RRF (n = 4/5). Unbiased stereological counting was performed by a blinded observer to estimate the number of DA neurons. Statistical analysis was carried out by two-way ANOVAs followed by Šidák’s corrections. Error bars represent ± SEM (ns, non-significant; *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001). Source data are provided as a Source Data file.

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