Fig. 3: The cellular composition of rejection characterized at the protein level using Multiple Iterative Labeling by Antibody Neodeposition (MILAN). | Nature Communications

Fig. 3: The cellular composition of rejection characterized at the protein level using Multiple Iterative Labeling by Antibody Neodeposition (MILAN).

From: Transcriptional and spatial profiling of the kidney allograft unravels a central role for FcyRIII+ innate immune cells in rejection

Fig. 3: The cellular composition of rejection characterized at the protein level using Multiple Iterative Labeling by Antibody Neodeposition (MILAN).The alternative text for this image may have been generated using AI.

a Experimental Approach. MILAN was used for characterization of infiltrating cells in kidney biopsies at the protein level in 18 kidney biopsies. Created using Biorender.com. b Representative sample illustrating the MILAN analysis with hematoxylin and eosin (H&E) staining (left), composite fluorescence image of 5 markers (plus autofluorescence, AF) after image processing (center), digital reconstruction of the sample highlighting the main cell populations of interest. c Uniform Manifold Approximation and Projection (UMAP) plot of 7804 representative cells. d Relative proportions of the 555,479 cells identified in the kidney transplant biopsies (n = 18). e Polar plot of the 18 biopsies. The biopsies were reclassified into 6 groups: non-rejecting donor-specific antibody (DSA) negative (NR DSA-) and DSA positive (NR DSA+), T-cell mediated rejection (TCMR) and antibody-mediated rejection DSA positive (ABMR DSA+) and DSA negative (ABMRh DSA-) and Mixed rejection. f Spearman’s correlation coefficient represented by a dot with 95% confident interval represented by the error bars of the correlation between inflammation severity and the frequency of different immune cells as a proportion of the total cell population (n = 18 biopsies). g Correlations between the frequency of indicated cell types and inflammation severity. Spearman’s correlation coefficient and two-tailed p-value are indicated.

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