Fig. 7: Transcriptional changes in FCGR3A+ NK and FCGR3A+ monocytes during rejection. | Nature Communications

Fig. 7: Transcriptional changes in FCGR3A+ NK and FCGR3A+ monocytes during rejection.

From: Transcriptional and spatial profiling of the kidney allograft unravels a central role for FcyRIII+ innate immune cells in rejection

Fig. 7

a, b The differentially expressed genes (DEGs) of FCGR3A+ cells were identified by the FindMarker function in Seurat using Wilcoxon test, according to the diagnostic phenotype of the samples (n = 16). a The fold change-fold change (FC-FC) plot compares the transcriptional differences within FCGR3A+ monocytes, between non-rejecting donor-specific antibody (NR DSA-) and NR DSA+ (y-axis) and antibody-mediated rejection (ABMR) (x-axis). The highlighted DEGs represent transcripts with log FC > 1.5 or log FC < −1.5 in ABMR cases compared to NR DSA-. b FC-FC plot comparing the DEGs in FCGR3A+ NK cells between NR DSA- and NR DSA+ (y axis) or ABMR (x axis). The highlighted DEGs represent transcripts with log FC > 1.5 or log FC < −1.5 in ABMR. c, d Dot plots demonstrating average gene expression values of selected genes (log scale), and percentage of FCGR3A+ Monocytes expressing indicated genes (c) or FCGR3A+ NK cells expressing indicated genes (d), according to inflammation severity (the radius on the polar plot22) represented by the rank of the biopsies in the plot.

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