Fig. 4: Identification of receptors in paracrine signaling by Click-IGC and Photo-IGC approaches.

a The secretome in conditioned media (CM) of KP4 cells were conjugated with probe 1, probe 2, and probe 3, respectively, and used to capture their receptors on HeLa cells. All experiments were performed in triplicate per condition. b Abundance of secreted proteins in KP4-CM. c Surfaceome analysis of Ac₄ManNAz labeled HeLa cells using CuAAC-based biotinylation. d Click-IGC with KP4-CM as ligands on HeLa cells. The UniProt annotated receptors and secreted proteins are highlighted in red and blue, respectively. e Gene Ontology annotations for cell localization of the significant changed proteins in Click-IGC and Photo-IGC experiments (Fig. 4d and Supplementary Fig. 10). f The significant changed cell surface and plasma membrane proteins in Fig. 4e and the corresponding potential ligand-receptor pairs. g The annotated cell surface receptors identified by different IGC methods. h Western blot validation of LIF as the signaling molecule responsible for the activation of STAT3 in HeLa cells. Images are representative of 3 biological replicates. Source data are provided as a Source Data file.