Fig. 5: Intracellular pH was measured with the pH-sensitive dye BCECF. | Nature Communications

Fig. 5: Intracellular pH was measured with the pH-sensitive dye BCECF.

From: Weak acids produced during anaerobic respiration suppress both photosynthesis and aerobic respiration

Fig. 5: Intracellular pH was measured with the pH-sensitive dye BCECF.The alt text for this image may have been generated using AI.

a Cells loaded with BCECF-AM using a confocal microscope (Leica TCS SP5). Fluorescence excitation was at 488 nm and emission at 515 ± 15 nm. Bar = 10 μm. Image of CC-400 cells, from left to right: BCECF fluorescence, chlorophyll fluorescence and the merged fluorescence. The experiment was performed in triplicate. b The intracellular pH calibration curve was measured by the excitation spectrum with emission at 530 nm. 50 mM NaAc (red diamond), 4.5 mM HAc (blue diamond) and untreated cells as a control (black diamond). The data points represent an average value from three independent measurements. The little black squares represent the averaged ratio of 490 nm to 439 nm at different pH values. c Model of “ion trap”: the dark green area represents the thylakoid, and the outer semicircle is the chloroplast. HA stands for the acetic acid neutral form, while A− for the acetate anion.

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