Fig. 4: The binding between 29 ACE2 orthologs and RBDs from BA.1, BA.2 and BA.4/5 detected by flow cytometry. | Nature Communications

Fig. 4: The binding between 29 ACE2 orthologs and RBDs from BA.1, BA.2 and BA.4/5 detected by flow cytometry.

From: Structural basis for receptor binding and broader interspecies receptor recognition of currently circulating Omicron sub-variants

Fig. 4: The binding between 29 ACE2 orthologs and RBDs from BA.1, BA.2 and BA.4/5 detected by flow cytometry.The alternative text for this image may have been generated using AI.

BHK-21 cells expressing eGFP-fused full-length ACE2s from 29 species, including humans, were incubated with BA.1, BA.2, and BA.4/5 RBDs with a His-tag in their C-terminus, respectively. SARS-CoV-2 PT NTD with a His-tag was used as the negative control for RBDs of BA.1, BA.2, and BA.4/5, and eGFP-fused full-length human CD26 was used as the negative control for ACE2s. The anti-His tag mouse monoclonal antibody conjugated to APC was used to detect His-tagged proteins. Three independent experiments were performed with similar results.

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