Fig. 1: PHF8 loss induces a growth-inhibiting immune response.
From: Loss of PHF8 induces a viral mimicry response by activating endogenous retrotransposons
a–d Tumor growth curves a, c and Kaplan-Meier survival curves b, d of Phf8 KO CT26 and MC38 cells and their corresponding vector control cells in immunodeficient mice. In each cases, about 500,000 (CT26) or 1,000,000 (MC38) tumor cells were inoculated subcutaneously into nude mice, and tumor formation was monitored. n = 7 tumors per group. e–h Tumor growth curves e, g and Kaplan-Meier survival curves f, h of Phf8 KO CT26 and MC38 cells and their corresponding vector control cells in syngeneic immunocompetent mice. n = 7 tumors per group. i, j Tumor growth curves i and Kaplan-Meier survival curves j of Balb/c mice subcutaneously injected with about 500,000 the vector control or Phf8 (exogenously transduced pLVX-Phf8 vector) CT26 cells. n = 6 tumors per group. k, l tumor growth curves k and Kaplan-Meier survival curves l of host mice after rechallenge with 1,000,000 Phf8 wild-type CT26 cells in Balb/c mice that remained tumor-free for 38 days after initial challenge with 500,000 Phf8 KO CT26 cells. Control referred to tumor-naive Balb/c mice challenged with Phf8 wild-type CT26 cells. n = 7 tumors per group. m Immunofluorescence staining (left) and quantitative data (right) of CD8+ (CD8+ T cells), granzyme B+ (GZMB+), interferon-γ+ (IFN-γ+) in the vector control and shPhf8 CT26 tumors. n = 5 biologically independent samples. Fluorescent density for each sample was analyzed. Immunohistochemical images shows Phf8 knockdown efficiency. Scale bar, 50 µm. n, o Tumor growth curves n and Kaplan-Meier survival curves o of Balb/c mice injected with 500,000 Phf8 wild-type CT26 cells that were treated with IgG control or anti-PD-1 antibodies, or the same number of shPhf8 CT26 cells treated with IgG control or anti-PD-1 antibodies. IgG or anti-PD-1 treatment began at day 8. n = 7 tumors per group. Data are presented as the mean ± sem. TF, tumor free. Two-way ANOVA in e, g, i, k, and n, log-rank test in f, h, l, and o, and unpaired two-sided Student’s t-test in m. Source data are provided as a Source Data file.
