Fig. 2: Multiplexed visual detection of fungal pathogens.

a Integration of each reaction of the assay using a paper-folding strategy. b Paper designs for detecting six wheat pathogenic fungi. c Multiplexed detection of six fungi using the colorimetric paper. d GPR values obtained by testing the 32 field-collected wheat leaf samples. e Comparison of results for detecting Pst infection in 32 wheat samples in (d) using the colorimetric assay and qPCR. Concentrations of DProbe, urease, urea, and phenol red were 100 nM, 1 nM, 500 mM and 250 μM. Source data are provided as a Source Data file.